Coding
NapA

Part:BBa_K4816009:Design

Designed by: Julia Alexander and Matias Brezina   Group: iGEM23_Bonn-Rheinbach   (2023-10-04)


Nitrate Reductase (napA) from Paracoccus denitrificans PD1222


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1003
    Illegal PstI site found at 127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1003
    Illegal PstI site found at 127
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1003
    Illegal BglII site found at 1208
    Illegal BglII site found at 1352
    Illegal BamHI site found at 534
    Illegal BamHI site found at 969
    Illegal BamHI site found at 1451
    Illegal BamHI site found at 2448
    Illegal XhoI site found at 2506
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1003
    Illegal PstI site found at 127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1003
    Illegal PstI site found at 127
    Illegal NgoMIV site found at 1302
    Illegal NgoMIV site found at 2065
    Illegal NgoMIV site found at 2130
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1041


Design Notes

Only active sites like napA needed for project, not the entire enzyme.


Source

Found on KEGG https://www.genome.jp/entry/pde:Pden_4721

References